rAAV-hSyn-mCherry-WPRE-hGH polyA
rAAV-hSyn-mCherry-WPRE-hGH polyA is a recombinant adeno-associated virus (rAAV) vector designed for targeted gene delivery. Here are the key components and features:
- Human Synapsin I Promoter (hSyn): Drives expression specifically in neurons.
- mCherry: A red fluorescent protein used for visualizing expression.
- WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element): Enhances transgene expression.
- hGH polyA (Human Growth Hormone Polyadenylation Signal): Ensures proper termination of transcription.
Application of rAAV-hSyn-mCherry-WPRE-hGH polyA
This rAAV vector is primarily used in neuroscience research for visualizing and studying neuronal activity. The hSyn promoter ensures neuron-specific expression, making it suitable for studies on neural circuits and behavior.
Advantage of rAAV-hSyn-mCherry-WPRE-hGH polyA
The use of the hSyn promoter ensures targeted expression in neurons, while the mCherry fluorescent protein allows for easy visualization of transduced cells. The inclusion of WPRE enhances the overall expression levels of the transgene, and the hGH polyA ensures proper transcription termination. This vector is a powerful tool for neuroimaging and functional studies, providing precise control over neuronal labeling with high specificity and efficiency.
Detailed Applications of rAAV-hSyn-mCherry-WPRE-hGH polyA
- Neuronal Labeling:
- Enables precise labeling of neurons for studying their morphology and connectivity.
- Suitable for tracing individual neurons and their processes in complex neural networks.
- Neuroscience Research:
- Facilitates the study of neuronal development, synaptic plasticity, and neural circuit dynamics.
- Useful for investigating the role of specific neuronal populations in behavior and brain function.
- Fluorescent Imaging:
- mCherry allows for visualization of infected neurons under a fluorescence microscope.
- Aids in verifying transduction efficiency and expression patterns.
- Behavioral Studies:
- Helps in linking specific neuronal activity to behavioral outcomes.
- Can be used to manipulate and observe changes in behavior following targeted neuronal labeling.
- Disease Models:
- Can be used to model neurological diseases where specific neuronal populations are affected.
- Useful in preclinical research for developing therapeutic strategies targeting specific neurons.
- Cell Tracking:
- Allows for tracking of neuronal migration and axonal growth in vivo.
- Facilitates studies on neuronal regeneration and repair following injury.