AffiAAV® rAAV-CAG-H2B-tdtomato-WPRE-hGH polyA

rAAV-CAG-H2B-tdTomato-WPRE-hGH polyA

rAAV-CAG-H2B-tdTomato-WPRE-hGH polyA is a recombinant adeno-associated virus (rAAV) vector designed for targeted gene delivery. Here are the key components and features:

• CAG Promoter: Drives high-level, ubiquitous expression across various cell types, including neurons.
• H2B (Histone H2B): Tags the fluorescent protein to histones, allowing for visualization of the nucleus.
• tdTomato: A red fluorescent protein used for visualizing expression.
• WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element): Enhances transgene expression.
• hGH polyA (Human Growth Hormone Polyadenylation Signal): Ensures proper termination of transcription.

Application of rAAV-CAG-H2B-tdTomato-WPRE-hGH polyA

This rAAV vector is primarily used in research for high-level expression and visualization of nuclear structures in a wide range of cell types and tissues. The CAG promoter ensures robust expression, making it suitable for various cell labeling and imaging applications.

Advantage of rAAV-CAG-H2B-tdTomato-WPRE-hGH polyA

The combination of H2B and tdTomato allows for precise visualization of nuclei, with the added advantage of high-level expression driven by the CAG promoter. The inclusion of WPRE enhances overall transgene expression, and the hGH polyA ensures proper transcription termination. This vector is a powerful tool for cellular and subcellular imaging, providing clear and detailed visualization of nuclear structures.

Detailed Applications of rAAV-CAG-H2B-tdTomato-WPRE-hGH polyA

1. Cell Labeling and Tracking:
   • Enables precise labeling of cell nuclei for tracking cell division, migration, and differentiation.
   • Suitable for studying dynamic cellular processes in live tissues.
2. Neuroscience Research:
   • Allows for visualization of neuronal nuclei, aiding in studies of neuronal development, connectivity, and function.
   • Useful for mapping neural circuits and understanding brain organization.
3. Developmental Biology:
   • Facilitates the study of nuclear dynamics during embryonic development.
   • Can be used to investigate the spatial and temporal patterns of cell division and differentiation.
4. Cancer Research:
   • Helps in visualizing and tracking cancer cell proliferation and metastasis.
   • Useful for studying the effects of anti-cancer treatments on tumor cells.
5. Histological Studies:
   • Provides a clear marker for nuclear structures in histological sections.
   • Aids in the analysis of tissue architecture and cellular organization.
6. Gene Expression Studies:
   • Allows for the study of gene expression patterns at the single-cell level.
   • Useful for investigating how gene expression correlates with nuclear morphology and positioning.