rAAV-CMV-EYFP-WPRE-hGH polyA
rAAV-CMV-EYFP-WPRE-hGH polyA is a recombinant adeno-associated virus (rAAV) vector designed for targeted gene delivery. Here are the key components and features:
- Cytomegalovirus Promoter (CMV): Drives strong, ubiquitous expression in a variety of cell types.
- EYFP (Enhanced Yellow Fluorescent Protein): Used for visualizing expression.
- WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element): Enhances transgene expression.
- hGH polyA (Human Growth Hormone Polyadenylation Signal): Ensures proper termination of transcription.
Application of rAAV-CMV-EYFP-WPRE-hGH polyA
This rAAV vector is primarily used in research for robust gene expression and visualization across a wide range of cell types and tissues. The CMV promoter ensures high levels of expression, making it suitable for various gene therapy and research applications.
Advantage of rAAV-CMV-EYFP-WPRE-hGH polyA
The combination of the CMV promoter and EYFP allows for high-level, ubiquitous expression of the transgene, with the added benefit of fluorescence for easy visualization. The inclusion of WPRE enhances the overall expression levels, and the hGH polyA ensures proper transcription termination. This vector is a versatile tool for gene expression studies, providing strong and widespread expression of the desired gene with high specificity and efficiency.
Detailed Applications of rAAV-CMV-EYFP-WPRE-hGH polyA
- Gene Therapy:
- Facilitates the delivery of therapeutic genes to a wide range of tissues.
- Suitable for treating genetic disorders by providing high levels of the therapeutic protein.
- Fluorescent Cell Labeling:
- EYFP allows for visualization of transduced cells under a fluorescence microscope.
- Useful for tracking and imaging specific cell populations in vivo and in vitro.
- Protein Production:
- Enables high-level production of recombinant proteins in various cell types.
- Useful for producing proteins for research, industrial, or therapeutic purposes.
- Functional Studies:
- Allows for the study of gene function by overexpressing the gene of interest in different cell types.
- Useful for investigating the effects of gene overexpression on cellular and physiological processes.
- Disease Models:
- Can be used to create models of diseases by overexpressing disease-related genes.
- Useful in preclinical research for understanding disease mechanisms and testing potential therapies.
- Developmental Biology:
- Facilitates the study of gene expression patterns during development.
- Useful for investigating the roles of specific genes in developmental processes.