AffiAAV® rAAV-EF1a-DIO-BFP-Flag-WPRE-bGH polyA

rAAV-EF1a-DIO-BFP-Flag-WPRE-bGH polyA

Overview:

This product is a recombinant adeno-associated virus (rAAV) designed for efficient gene delivery. The construct includes a Blue Fluorescent Protein (BFP) and a Flag tag under the control of the EF1a promoter, making it ideal for dual-activity monitoring and protein expression studies.

Key Features:

• High-efficiency gene delivery: The rAAV system allows for precise targeting and delivery of genetic material.
• Dual expression: The BFP allows for visual tracking, while the Flag tag facilitates easy detection and purification.
• EF1a promoter: A strong, ubiquitous promoter for high expression levels across various cell types.
• WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element): Enhances gene expression efficiency.

• BGH PolyA: Ensures proper mRNA processing and stability.

Applications:

• Gene expression studies: Monitor gene delivery and expression through BFP fluorescence.
• Protein tracking and purification: The Flag tag allows for easy antibody-based detection and purification of the expressed protein.
• In vivo and in vitro experiments: Suitable for a range of applications, from cell cultures to live animal studies.

Technical Specifications:

• Promoter: EF1a (Ubiquitous expression)
• Reporter: BFP (Blue Fluorescent Protein)
• Tag: Flag (For protein detection and purification)

• Regulatory Elements: WPRE, bGH PolyA

Storage and Handling:

• Store at -80°C.
• Thaw on ice before use.

• Avoid repeated freeze-thaw cycles.

References:

For detailed protocols and more information on how to use this vector, visit our [resource page] or contact our technical support team.