AffiAAV® scAAV-hSyn-fDIO-EGFP-WPREs

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CAT# AFG-BRV-0273
Size: 1 Unit

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scAAV-hSyn-fDIO-EGFP-WPREs

scAAV-hSyn-fDIO-EGFP-WPREs is a self-complementary recombinant adeno-associated virus (scAAV) vector designed for targeted gene delivery. Here are the key components and features:

  • Human Synapsin I Promoter (hSyn): Drives expression specifically in neurons.
  • fDIO (flip-excision (FLEx) Double-floxed Inverted Open reading frame): Allows Cre-dependent expression of the transgene.
  • EGFP (Enhanced Green Fluorescent Protein): Used for visualizing expression.
  • WPREs (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Elements): Enhance transgene expression.

Application of scAAV-hSyn-fDIO-EGFP-WPREs

This scAAV vector is primarily used in neuroscience research for conditional and cell-type-specific expression of the EGFP protein in neurons in the presence of Cre recombinase. The hSyn promoter ensures neuron-specific expression, making it suitable for studies on neural circuits and behavior.


Advantage of scAAV-hSyn-fDIO-EGFP-WPREs

The combination of the hSyn promoter and fDIO system allows for Cre-dependent expression of the EGFP protein, ensuring precise control over gene expression in neurons. The self-complementary nature of the vector allows for faster and higher expression levels, and the inclusion of WPREs enhances overall transgene expression. This vector is a powerful tool for targeted neuroimaging and functional studies, providing high specificity and efficiency.


Detailed Applications of scAAV-hSyn-fDIO-EGFP-WPREs

  1. Conditional Gene Expression:
    • Enables Cre-dependent expression of EGFP, allowing for precise temporal and spatial control of gene expression in neurons.
    • Suitable for studying the effects of gene activation in specific neuronal populations.
  2. Fluorescent Cell Labeling:
    • EGFP allows for visualization of transduced neurons under a fluorescence microscope.
    • Useful for tracking and imaging specific neuronal populations in vivo and in vitro.
  3. Neuroscience Research:
    • Facilitates the study of neural circuits and their role in behavior and brain function.
    • Helps in mapping and understanding the connectivity and function of neurons.
  4. Neural Circuit Mapping:
    • Allows for tracing and understanding the connections and interactions between neurons.
    • Combined with other techniques, it can reveal functional connectivity in the brain.
  5. Behavioral Studies:
    • Helps in linking specific neuronal activity to behavioral outcomes.
    • Can be used to manipulate and observe changes in behavior following targeted neuronal labeling.
  6. Disease Models:
    • Can be used to model neurological diseases where specific neuronal populations are affected.
    • Useful in preclinical research for developing therapeutic strategies targeting neurons.

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