scAAV-hSyn-fDIO-EGFP-WPREs
scAAV-hSyn-fDIO-EGFP-WPREs is a self-complementary recombinant adeno-associated virus (scAAV) vector designed for targeted gene delivery. Here are the key components and features:
- Human Synapsin I Promoter (hSyn): Drives expression specifically in neurons.
- fDIO (flip-excision (FLEx) Double-floxed Inverted Open reading frame): Allows Cre-dependent expression of the transgene.
- EGFP (Enhanced Green Fluorescent Protein): Used for visualizing expression.
- WPREs (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Elements): Enhance transgene expression.
Application of scAAV-hSyn-fDIO-EGFP-WPREs
This scAAV vector is primarily used in neuroscience research for conditional and cell-type-specific expression of the EGFP protein in neurons in the presence of Cre recombinase. The hSyn promoter ensures neuron-specific expression, making it suitable for studies on neural circuits and behavior.
Advantage of scAAV-hSyn-fDIO-EGFP-WPREs
The combination of the hSyn promoter and fDIO system allows for Cre-dependent expression of the EGFP protein, ensuring precise control over gene expression in neurons. The self-complementary nature of the vector allows for faster and higher expression levels, and the inclusion of WPREs enhances overall transgene expression. This vector is a powerful tool for targeted neuroimaging and functional studies, providing high specificity and efficiency.
Detailed Applications of scAAV-hSyn-fDIO-EGFP-WPREs
- Conditional Gene Expression:
- Enables Cre-dependent expression of EGFP, allowing for precise temporal and spatial control of gene expression in neurons.
- Suitable for studying the effects of gene activation in specific neuronal populations.
- Fluorescent Cell Labeling:
- EGFP allows for visualization of transduced neurons under a fluorescence microscope.
- Useful for tracking and imaging specific neuronal populations in vivo and in vitro.
- Neuroscience Research:
- Facilitates the study of neural circuits and their role in behavior and brain function.
- Helps in mapping and understanding the connectivity and function of neurons.
- Neural Circuit Mapping:
- Allows for tracing and understanding the connections and interactions between neurons.
- Combined with other techniques, it can reveal functional connectivity in the brain.
- Behavioral Studies:
- Helps in linking specific neuronal activity to behavioral outcomes.
- Can be used to manipulate and observe changes in behavior following targeted neuronal labeling.
- Disease Models:
- Can be used to model neurological diseases where specific neuronal populations are affected.
- Useful in preclinical research for developing therapeutic strategies targeting neurons.