rAAV-CMV-rox-stop-rox-mCherry-WPREs Overview
rAAV-CMV-rox-stop-rox-mCherry-WPREs is a recombinant adeno-associated virus (rAAV) vector designed for targeted gene delivery. Here are the key components and features:
- Cytomegalovirus Promoter (CMV):
- Function: Drives strong, ubiquitous expression in a variety of cell types.
- Applications: Suitable for high-level gene expression in numerous biological systems.
- rox-stop-rox:
- Function: A rox-flanked stop cassette allowing Dre-dependent expression of the transgene.
- Applications: Provides precise control over gene expression, activated only in the presence of Dre recombinase.
- mCherry:
- Function: A red fluorescent protein for visualizing gene expression.
- Applications: Enables tracking and imaging of transduced cells.
- Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element (WPRE):
- Function: Enhances transgene expression by improving mRNA stability and translation.
- Applications: Increases overall efficiency and effectiveness of gene expression.
Application of rAAV-CMV-rox-stop-rox-mCherry-WPREs
This rAAV vector is primarily used in research for conditional and cell-type-specific expression of the mCherry protein in the presence of Dre recombinase. The CMV promoter ensures robust expression, making it suitable for various gene expression studies and cell labeling applications.
Advantages of rAAV-CMV-rox-stop-rox-mCherry-WPREs
The combination of the CMV promoter and rox system allows for Dre-dependent expression of the mCherry protein, ensuring precise control over gene expression. The WPRE enhances overall transgene expression, making this vector a versatile tool for conditional gene expression studies, providing high specificity and efficiency.
Detailed Applications of rAAV-CMV-rox-stop-rox-mCherry-WPREs
Conditional Gene Expression:
- Enables Dre-dependent expression of mCherry, allowing for precise temporal and spatial control of gene expression.
- Suitable for studying the effects of gene activation in specific cell populations.
Fluorescent Cell Labeling:
- mCherry allows for visualization of transduced cells under a fluorescence microscope.
- Useful for tracking and imaging specific cell populations in vivo and in vitro.
Neuroscience Research:
- Can be used to study neural circuits and behavior by selectively expressing mCherry in Dre-expressing neurons.
- Helps in mapping and understanding neural connectivity and function.
Developmental Biology:
- Allows for conditional expression of genes during specific stages of development.
- Useful for investigating the roles of specific genes in developmental processes.
Disease Models:
- Can be used to model diseases by conditionally expressing disease-related genes in specific cell types.
- Useful in preclinical research for developing therapeutic strategies targeting specific cell populations.