AffiAAV® rAAV-OT-Flp-WPREs

rAAV-OT-Flp-WPREs

rAAV-OT-Flp-WPREs is a recombinant adeno-associated virus (rAAV) vector designed for targeted gene delivery. Here are the key components and features:

• Oxytocin (OT) Promoter: Drives expression specifically in oxytocin-expressing neurons.
• Flp Recombinase: An enzyme that facilitates site-specific recombination at FRT sites, enabling targeted gene modification.
• Woodchuck Hepatitis Virus Post-transcriptional Regulatory Elements (WPREs): Enhances transgene expression and stability.

Application of rAAV-OT-Flp-WPREs

This rAAV vector is primarily used in neuroscience research for targeted genetic manipulation of oxytocin-expressing neurons. The OT promoter ensures specific expression in these neurons, making it suitable for studies on neural circuits, social behavior, and neuroendocrine functions.

Advantage of rAAV-OT-Flp-WPREs

The combination of the OT promoter and Flp recombinase allows for precise genetic manipulation of oxytocin-expressing neurons. The inclusion of WPREs enhances the overall stability and expression levels of the transgene. This vector is a powerful tool for studies requiring targeted gene modification with high specificity and efficiency.

Detailed Applications of rAAV-OT-Flp-WPREs

Targeted Genetic Modification:

• Enables specific gene modification in oxytocin-expressing neurons using Flp-FRT recombination.
• Suitable for creating conditional knockout or knock-in models in these specific neurons.

Neural Circuit Mapping:

• Helps in mapping and understanding the role of oxytocin-expressing neurons in neural circuits.
• Can be used in conjunction with other techniques to reveal functional connectivity in the brain.

Behavioral Studies:

• Facilitates the study of how oxytocin-expressing neurons influence social behavior and other related behaviors.
• Can be used to investigate the neural basis of various social phenomena and neuroendocrine functions.

Disease Models:

• Useful in modeling neurological and psychiatric disorders where oxytocin-expressing neurons play a role.
• Can aid in preclinical research for developing therapeutic strategies targeting these specific neurons.

Gene Regulation Studies:

• Flp recombinase can be used for gene regulation studies, allowing for controlled gene expression and manipulation.
• Provides a tool for studying the effects of gene deletion or activation in specific neuronal populations.

Fluorescent Imaging (when combined with reporter lines):

• When used with Flp-reporter lines, it allows for visualization of infected neurons under a fluorescence microscope.
• Aids in verifying transduction efficiency and expression patterns.

This vector is an invaluable resource for researchers aiming to manipulate specific neuronal populations with precision, providing a robust platform for various genetic and functional studies in neuroscience.