AffiAAV® rAAV-mTH-ArchT-mCherry-WPREs

rAAV-mTH-ArchT-mCherry-WPREs

rAAV-mTH-ArchT-mCherry-WPREs is a recombinant adeno-associated virus (rAAV) vector designed for targeted gene delivery. Here are the key components and features:

• Mouse Tyrosine Hydroxylase Promoter (mTH): Drives expression specifically in dopaminergic neurons.
• ArchT (Archaerhodopsin-3): Provides optogenetic silencing capability.
• mCherry: Fluorescent protein fused with ArchT for visualization of expression.
• WPREs (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Elements): Enhance transgene expression.

Application of rAAV-mTH-ArchT-mCherry-WPREs

This rAAV vector is primarily used in neuroscience research for optogenetic silencing of dopaminergic neurons. The mTH promoter ensures neuron-specific expression, making it suitable for studies on dopaminergic neural circuits and behavior.

Advantage of rAAV-mTH-ArchT-mCherry-WPREs

The combination of ArchT and mCherry allows for light-driven silencing of neurons, with the added advantage of visualizing expression through mCherry fluorescence. The inclusion of WPREs enhances the overall expression levels of the transgene, and the use of the mTH promoter ensures targeted expression in dopaminergic neurons. This vector is a powerful tool for optogenetic studies, providing precise control over neuronal activity with high specificity and efficiency.

Detailed Applications of rAAV-mTH-ArchT-mCherry-WPREs

1. Optogenetic Silencing:
   • Enables precise control over neuronal silencing using green light.
   • Suitable for studying the role of dopaminergic neurons in various brain functions and behaviors.
2. Neural Circuit Mapping:
   • Helps in tracing and understanding dopaminergic neural circuits by selectively silencing neurons.
   • Combined with other techniques, it can reveal functional connectivity in the brain.
3. Behavioral Studies:
   • Facilitates the study of how specific dopaminergic neurons and circuits influence behavior.
   • Can be used to investigate the neural basis of various behavioral phenomena and neurological disorders.
4. Fluorescent Imaging:
   • mCherry allows for visualization of infected neurons under a fluorescence microscope.
   • Aids in verifying transduction efficiency and expression patterns.
5. Disease Models:
   • Can be used to model neurological diseases where aberrant dopaminergic neuronal activity plays a role.
   • Useful in preclinical research for developing therapeutic strategies targeting specific neuronal populations.