AffiAAV® rAAV-CAG-FLEX-ArchT-EGFP-WPRE-SV40 polyA

rAAV-CAG-FLEX-ArchT-EGFP-WPRE-SV40 polyA

rAAV-CAG-FLEX-ArchT-EGFP-WPRE-SV40 polyA is a recombinant adeno-associated virus (rAAV) vector designed for targeted gene delivery. Here are the key components and features:

• CAG Promoter: Drives high-level, ubiquitous expression across various cell types, including neurons.
• FLEX (flip-excision (FLEx) Double-floxed Inverted Open reading frame): Allows Cre-dependent expression of the transgene.
• ArchT (Archaerhodopsin-3): Provides optogenetic silencing capability.
• EGFP (Enhanced Green Fluorescent Protein): Fused with ArchT for visualization of expression.
• WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element): Enhances transgene expression.
• SV40 polyA (Simian Virus 40 Polyadenylation Signal): Ensures proper termination of transcription.

Application of rAAV-CAG-FLEX-ArchT-EGFP-WPRE-SV40 polyA

This rAAV vector is primarily used in neuroscience research for conditional and cell-type-specific expression of the ArchT-EGFP protein in the presence of Cre recombinase. The CAG promoter ensures robust expression, making it suitable for studies on neural circuits and behavior.

Advantage of rAAV-CAG-FLEX-ArchT-EGFP-WPRE-SV40 polyA

The combination of the CAG promoter and FLEX system allows for Cre-dependent expression of the ArchT-EGFP protein, ensuring precise control over gene expression. The ArchT component provides optogenetic silencing capability, while EGFP allows for easy visualization of transduced cells. The inclusion of WPRE enhances overall transgene expression, and the SV40 polyA ensures proper transcription termination. This vector is a powerful tool for targeted neuroimaging and functional studies, providing high specificity and efficiency.

Detailed Applications of rAAV-CAG-FLEX-ArchT-EGFP-WPRE-SV40 polyA

1. Optogenetic Silencing:
   • Enables precise control over neuronal silencing using green light.
   • Suitable for studying the role of specific neurons in various brain functions and behaviors.
2. Conditional Gene Expression:
   • Enables Cre-dependent expression of ArchT-EGFP, allowing for precise temporal and spatial control of gene expression in specific cell types.
   • Suitable for studying the effects of gene activation in specific neuronal populations.
3. Fluorescent Cell Labeling:
   • EGFP allows for visualization of transduced cells under a fluorescence microscope.
   • Useful for tracking and imaging specific cell populations in vivo and in vitro.
4. Neuroscience Research:
   • Facilitates the study of neural circuits and their role in behavior and brain function.
   • Helps in mapping and understanding the connectivity and function of neurons.
5. Neural Circuit Mapping:
   • Allows for tracing and understanding the connections and interactions between neurons.
   • Combined with other techniques, it can reveal functional connectivity in the brain.
6. Behavioral Studies:
   • Helps in linking specific neuronal activity to behavioral outcomes.
   • Can be used to manipulate and observe changes in behavior following targeted neuronal silencing.
7. Disease Models:
   • Can be used to model neurological diseases where specific neuronal populations are affected.
   • Useful in preclinical research for developing therapeutic strategies targeting specific neurons.