Overview of rAAV-CMV

Recombinant Adeno-Associated Virus (rAAV) vectors are pivotal in gene therapy and various research domains for their efficiency in delivering genetic material into host cells. The rAAV-CMV vector employs the Cytomegalovirus (CMV) promoter, known for its strong and ubiquitous expression, making it suitable for studies requiring high levels of transgene expression across a wide range of cell types.

Technical Content on rAAV-CMV

Vector Construction:

  • The rAAV-CMV vector integrates the CMV promoter upstream of the gene of interest.
  • The CMV promoter is derived from the human Cytomegalovirus immediate-early gene, which is recognized for its potent transcriptional activity in many cell types.
  • The vector construction ensures robust and sustained expression of the transgene, essential for applications requiring high gene expression.

Mechanism of Action:

  • Upon delivery into the host cell, the rAAV vector utilizes the host cell’s transcriptional machinery to drive transgene expression under the control of the CMV promoter.
  • The CMV promoter's strong enhancer elements recruit a wide array of transcription factors, facilitating efficient transcription initiation.
  • The ubiquitous nature of the CMV promoter ensures that the transgene is expressed in a broad spectrum of cell types, both in vitro and in vivo.

Applications:

  • Gene Therapy: rAAV-CMV vectors are widely used to deliver therapeutic genes for various genetic disorders. The high expression levels driven by the CMV promoter are advantageous for conditions requiring substantial amounts of the gene product.
  • Neuroscience Research: The vector is utilized to overexpress genes in neuronal and glial cells, aiding studies of brain function, neural circuits, and disease mechanisms.
  • Cancer Research: rAAV-CMV vectors are employed to express genes in cancer cells, facilitating research into cancer biology and potential gene-based treatments.
  • Protein Production: The vector is used in cell culture systems to produce recombinant proteins, which can be harvested and used for various biochemical and therapeutic purposes.

Advantages:

  • Strong Expression: The CMV promoter drives robust transgene expression, ensuring high levels of gene product necessary for therapeutic and research applications.
  • Broad Tropism: The promoter is active in a wide range of cell types, making the vector versatile for different experimental models and therapeutic targets.
  • Efficient Transduction: rAAV vectors efficiently transduce both dividing and non-dividing cells, ensuring widespread gene delivery and expression.

Challenges:

  • Immune Response: The strong expression driven by the CMV promoter can sometimes lead to an immune response, particularly in vivo, which may affect the duration and stability of transgene expression.
  • Silencing: In some contexts, the CMV promoter can be prone to silencing, especially in certain cell types or with long-term expression, which can limit its effectiveness.
  • Vector Production: High-titer production of rAAV-CMV vectors requires optimized packaging and purification processes to achieve sufficient yields for both experimental and therapeutic applications.
AffiAAV® rAAV-CMV-rox-stop-rox-mCherry-WPREs
CAT# AFG-BRV-0279
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0250
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0249
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0248
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-CMV-DIO-3XFlag-WPREs
CAT# AFG-BRV-0246
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-Flag-WPREs
CAT# AFG-BRV-0245
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0244
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DO-EGFP-WPREs
CAT# AFG-BRV-0185
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0074
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-mRuby3-WPREs
CAT# AFG-BRV-0073
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-3×Flag-WPRE-hGH polyA
CAT# AFG-BRV-0072
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EGFP-WPREs
CAT# AFG-BRV-0071
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0069
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0068
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-HA-WPRE-hGH polyA
CAT# AFG-BRV-0067
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-mcherry-WPRE-hGH polyA
CAT# AFG-BRV-0066
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0065
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EGFP-WPRE-bGH polyA
CAT# AFG-BRV-0064
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Flag-Flag-WPREs
CAT# AFG-BRV-0063
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EGFP -WPRE-bGH polyA
CAT# AFG-BRV-0062
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Flag-WPREs
CAT# AFG-BRV-0061
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-WPRE-hGH polyA
CAT# AFG-BRV-0060
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-mCherry-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-0059
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EGFP-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-0057
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-QuasAr2-WPRE-hGHpolyA
CAT# AFG-BRV-1361
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1077
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Flp-hGH polyA
CAT# AFG-BRV-1076
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EGFP-P2A-Cre-WPRE-bGH polyA
CAT# AFG-BRV-0932
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Cre-Flag-WPREs
CAT# AFG-BRV-0931
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Cre-plus-WPRE-bGH polyA
CAT# AFG-BRV-0929
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Cre-WPRE-hGH polyA
CAT# AFG-BRV-0928
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Cre-hGH polyA
CAT# AFG-BRV-0927
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-NES-jRGECO1a-WPRE-hGH polyA
CAT# AFG-BRV-0888
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-jGCaMP7s-WPRE-hGH polyA
CAT# AFG-BRV-0860
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-GCaMP6s-ER/K-tdtomato-wpres
CAT# AFG-BRV-0821
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-GCaMp6s-WPRE-bGH polyA
CAT# AFG-BRV-0766
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-GCaMp6f-WPRE-hGH  polyA
CAT# AFG-BRV-0742
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-hM3D(Gq)-mCherry-WPREs
CAT# AFG-BRV-0580
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Snca-P2A-EGFP-WPREs
CAT# AFG-BRV-0567
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-SYN-SNCA(A53T)-WPRE-bGHpA
CAT# AFG-BRV-0556
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-SYN-SNCA(A30P)-WPRE-bGHpA
CAT# AFG-BRV-0555
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-SYN-SNCA(WT)-WPRE-bGHpA
CAT# AFG-BRV-0554
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-SYN-EGFP-WPRE-bGHpA
CAT# AFG-BRV-0553
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-htau-mCherry-MCS-WPRE
CAT# AFG-BRV-0541
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-tau(P301L)-EGFP-WPREs
CAT# AFG-BRV-0540
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-htau-mCherry-WPREs
CAT# AFG-BRV-0538
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-htau-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0537
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-tau-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0536
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-mCherry-tau-WPRE-hGH polyA
CAT# AFG-BRV-0535
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-EGFP-Tau-bGH polyA
CAT# AFG-BRV-0532
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-TagBFP-P2A-oRVG-WPREs
CAT# AFG-BRV-0431
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-oRVG-WPRE-hGH polyA
CAT# AFG-BRV-0430
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-ert2Creert2-mCherry-WPREs
CAT# AFG-BRV-0372
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-rtTA-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0360
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-rTTA-WPRE-hGH polyA
CAT# AFG-BRV-0358
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-tTA-WPRE-hGH polyA
CAT# AFG-BRV-0348
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD