rAAV-nEF1a

The nEF1α promoter (short for "neuron-specific elongation factor 1 alpha promoter") is a modified version of the EF1α promoter designed for neuron-specific transgene expression. Recombinant adeno-associated viruses (rAAVs) incorporating the nEF1α promoter are ideal for applications requiring robust and long-term gene expression restricted to neurons.

Features of rAAV-nEF1α

• Neuron-Specific Expression:
  • The nEF1α promoter restricts transgene expression to neurons while avoiding expression in non-neuronal cells like glia or peripheral tissues.
• Strong and Stable Expression:
  • Provides robust transgene expression comparable to general EF1α promoters but limited to neuronal populations.
• Long-Term Expression:
  • Suitable for chronic studies due to stable transgene expression in vivo.
• Cross-Species Compatibility:
  • Functional across diverse mammalian species, including rodents, non-human primates, and humans.

Applications of rAAV-nEF1α

• Neuroscience Research:
  • Circuit Mapping: Delivery of fluorescent markers or tracers to investigate neuronal connectivity.
  • Optogenetics and Chemogenetics: Expression of optogenetic tools (e.g., channelrhodopsins) or chemogenetic receptors (e.g., DREADDs) for neuronal manipulation.
  • Calcium Imaging: Use of genetically encoded calcium indicators (e.g., GCaMP) to monitor neuronal activity.
• Gene Therapy:
  • Delivery of therapeutic genes for neurodegenerative diseases (e.g., Parkinson's, Alzheimer's) and other CNS disorders with specificity to neurons.
• Behavioral Studies:
  • Targeted manipulation of neuronal populations to study their role in sensory processing, memory, and behavior.
• CRISPR/Cas9 Genome Editing:
  • Delivery of Cas9 and guide RNAs to neurons for targeted gene modification.

rAAV-nEF1α is a highly effective and specific tool for neuronal gene delivery, providing strong, stable, and long-term transgene expression in neurons. Its versatility and neuron-specific targeting make it a preferred choice for applications in neuroscience research and therapeutic development.