rAAV-TRE3g

The TRE3g promoter (tetracycline-responsive element version 3, enhanced) is a component of the Tet-On/Tet-Off system, which enables inducible and tightly controlled gene expression. Recombinant adeno-associated viruses (rAAVs) incorporating TRE3g promoters allow for doxycycline-dependent transgene regulation, providing precise temporal control over gene expression.

Features of rAAV-TRE3g
  • Inducible Gene Expression:
    • TRE3g requires the presence of the rtTA (reverse tetracycline transactivator) protein and doxycycline to initiate transcription. Expression ceases when doxycycline is removed.
    • Tight on/off control ensures minimal leaky expression in the absence of doxycycline.
  • Enhanced Sensitivity:
    • TRE3g is an improved version of earlier TRE promoters, offering reduced background expression and higher responsiveness to doxycycline.
  • Temporal Control:
    • Enables precise timing of transgene expression, critical for applications where constitutive expression is undesirable.
  • Compatibility with Diverse Systems:
    • Can be combined with neuron-, glia-, or tissue-specific promoters driving rtTA expression for cell-type-specific and inducible transgene control.
Applications of rAAV-TRE3g
  • Neuroscience Research:
    • Circuit Manipulation: Inducible delivery of optogenetic or chemogenetic tools (e.g., channelrhodopsins, DREADDs) to specific neural circuits.
    • Calcium Imaging: Temporal control over calcium indicators (e.g., GCaMP) for activity-dependent studies.
  • Gene Therapy:
    • Therapeutic Regulation: Controlled delivery of therapeutic genes, allowing regulation of dosage and timing in diseases like epilepsy or neurodegeneration.
    • Safety: Reduces risks associated with constitutive expression of potent transgenes by enabling doxycycline-dependent expression.
  • Disease Modeling:
    • Inducible models of disease that require temporally regulated gene expression for specific developmental stages or experimental conditions.
  • CRISPR/Cas9 Genome Editing:
    • Controlled activation of CRISPR/Cas9 systems to minimize off-target effects or study time-sensitive genetic interventions.

rAAV-TRE3g is an advanced tool for precise, inducible gene expression, offering robust control over timing and dosage. It is especially useful in applications requiring temporal regulation of gene expression, such as functional studies, disease modeling, and therapeutic development. When combined with tissue-specific rtTA expression, it provides unparalleled flexibility in gene delivery and regulation.

AffiAAV® rAAV-TRE3g-H2B-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0153
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-tdTomato-WPRE-hGH polyA
CAT# AFG-BRV-0152
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0151
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0150
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-eNpHR3.0-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-1298
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-TRE3g-DIO-hChR2(H134R)-YFP-WPREs
CAT# AFG-BRV-1176
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-TRE3g-EYFP-P2A-Cre-WPRE-hGH polyA
CAT# AFG-BRV-0964
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3G-Cre-WPRE-hGH polyA
CAT# AFG-BRV-0963
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-DIO-CaMPARI-WPRE-hGH polyA
CAT# AFG-BRV-0876
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-jGCaMP7s-WPRE-hGH polyA
CAT# AFG-BRV-0853
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-DIO-GCaMp6f-WPRE-hGH polyA
CAT# AFG-BRV-0747
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-DIO-GCaMP6M-WPRE-hGH polyA
CAT# AFG-BRV-0746
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-hM4D(Gi)-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0714
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-TRE3g-EGFP-T2A-TVA-WPREs
CAT# AFG-BRV-0513
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-TRE3g-DIO-GCaMP6M-WPRE-hGH polyA
CAT# AFG-BRV-0317
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-DIO-GCaMp6f-WPRE-hGH polyA
CAT# AFG-BRV-0316
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-jGCaMP7s-WPRE-hGH polyA
CAT# AFG-BRV-0315
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-eNpHR3.0-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0314
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-NpHR3.0-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0313
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-DIO-hChR2(H134R)-YFP-WPREs
CAT# AFG-BRV-0309
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-Cre-P2A-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0305
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3G-Cre-WPRE-hGH polyA
CAT# AFG-BRV-0304
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-EGFP-T2A-TVA-WPREs
CAT# AFG-BRV-0301
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-TRE3g-hM4D(Gi)-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0293
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-H2B-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0287
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-tdTomato-WPRE-hGH polyA
CAT# AFG-BRV-0286
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-CMV-H2B-mCherry-pA
CAT# AFG-BRV-0284
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0283
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0282
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE3g-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0281
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD