rAAV-EF1a

Recombinant adeno-associated viruses (rAAVs) utilizing the EF1α promoter are designed for robust, constitutive expression of transgenes in a wide range of cell types. The elongation factor 1 alpha (EF1α) promoter is derived from a highly conserved eukaryotic gene involved in protein synthesis, making it an excellent choice for high and stable expression across various experimental models.

Features of rAAV-EF1α
  • Broad Tropism:
    • The EF1α promoter drives transgene expression in multiple cell types, including neurons, glia, muscle, and other somatic cells.
    • Effective in both dividing and non-dividing cells.
  • Species Compatibility:
    • Functional across a broad range of mammalian species, including rodents, primates, and humans.
  • Stable and Long-Term Expression:
    • Supports sustained expression in vivo, ideal for chronic studies.
  • Moderate to High Expression Levels:
    • Provides strong expression, though generally lower than the CMV promoter, with reduced risk of promoter silencing in certain tissues.
Applications of rAAV-EF1α
  • Gene Therapy:
    • Delivery of therapeutic genes for a wide range of diseases, such as muscular dystrophies, neurodegenerative disorders, and retinal diseases.
  • Neuroscience Research:
    • Expression of optogenetic and chemogenetic tools for circuit manipulation.
    • Delivery of fluorescent reporters (e.g., GFP, RFP) for imaging and cell tracking.
  • CRISPR/Cas9 and Genome Editing:
    • Delivery of gene-editing components, such as Cas9, guide RNAs, or base editors, in diverse cell types.
  • Molecular and Cellular Studies:
    • Overexpression of proteins or knockdown of genes to study their roles in biological processes.
    • Use in stem cell research and organoid models.
  • Animal Models:
    • Generating transgenic animal models for disease research.

rAAV-EF1α is a versatile and reliable gene delivery system suitable for a wide range of research and therapeutic applications. Its broad applicability and stability make it a preferred choice for experiments requiring constitutive transgene expression in diverse cell populations.

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AffiAAV® rAAV-EF1a-fDIO-tdTomato-WPRE-hGH polyA
CAT# AFG-BRV-0268
Size: 1 Unit
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AffiAAV® rAAV-EF1a-fDIO-axon-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0267
Size: 1 Unit
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AffiAAV® rAAV-EF1a-fDIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0266
Size: 1 Unit
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AffiAAV® rAAV-EF1a-fDIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0265
Size: 1 Unit
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AffiAAV® rAAV-EF1a-fDIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0264
Size: 1 Unit
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AffiAAV® rAAV-EF1a-fDIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0263
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-H2B-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0232
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-3XFlag-P2A-EGFP-WPREs
CAT# AFG-BRV-0230
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-ZsGreen1-WPREs
CAT# AFG-BRV-0229
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0228
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-H2B-Dsred-WPRE-hGH polyA
CAT# AFG-BRV-0227
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-3XFlag-WPREs
CAT# AFG-BRV-0226
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-3XMyc-WPRE-hGH polyA
CAT# AFG-BRV-0225
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-3XHA-WPRE-hGH polyA
CAT# AFG-BRV-0224
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-0223
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-Flag-WPREs
CAT# AFG-BRV-0218
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-Flag-WPREs
CAT# AFG-BRV-0217
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-H2B-EGFP-WPRE-pA
CAT# AFG-BRV-0216
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0215
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-EGFP-SV40 pA
CAT# AFG-BRV-0214
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0211
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-BFP-Flag-WPRE-bGH polyA
CAT# AFG-BRV-0210
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DO-tdTomato-WPRE-hGH polyA
CAT# AFG-BRV-0184
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DO-Dsred-WPRE-hGH polyA
CAT# AFG-BRV-0183
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0182
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0181
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0180
Size: 1 Unit
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AffiAAV® rAAV-EF1a-WPRE-hGH polyA
CAT# AFG-BRV-0017
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DsRed-WPRE-hGH polyA
CAT# AFG-BRV-0016
Size: 1 Unit
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AffiAAV® rAAV-EF1a-IRES-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0013
Size: 1 Unit
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AffiAAV® rAAV-EF1a-EGFP-SV40 pA
CAT# AFG-BRV-0012
Size: 1 Unit
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AffiAAV® rAAV-EF1a-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0011
Size: 1 Unit
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AffiAAV® rAAV-EF1a-con fon-Jaws-EYFP-ER2-WPREs
CAT# AFG-BRV-1359
Size: 1 Unit
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AffiAAV® rAAV-EF1a-Con Fon-ArchT-EGFP-WPREs
CAT# AFG-BRV-1353
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-ArCH-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1344
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-FLlnChR-EYFP-WPRE-hGHpA
CAT# AFG-BRV-1334
Size: 1 Unit
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AffiAAV® rAAV-EF1a-eNpHR3.0-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1281
Size: 1 Unit
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AffiAAV® rAAV-EF1a-eNpHR3.0-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-1279
Size: 1 Unit
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AffiAAV® rAAV-EF1a-fDIO-chrimsonR-mCherry-WPREs
CAT# AFG-BRV-1270
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-C1V1-EGFP-WPRE-hGH pA
CAT# AFG-BRV-1252
Size: 1 Unit
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AffiAAV® rAAV-EF1a-C1V1-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-1248
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-ChETA-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-1206
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DO-hChR2(H134R)-mCherry-WPREs
CAT# AFG-BRV-1178
Size: 1 Unit
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AffiAAV® rAAV-EF1a-ChR2-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-1118
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-CheRiff-EYFP2
CAT# AFG-BRV-1117
Size: 1 Unit
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AffiAAV® rAAV-EF1a-HA-Dre-WPRE-hGH polyA
CAT# AFG-BRV-1115
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-Flp-plus-WPRE-hGH polyA
CAT# AFG-BRV-1087
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-Flp-WPREs
CAT# AFG-BRV-1082
Size: 1 Unit
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AffiAAV® rAAV-EF1a-fDIO-Cre-WPREs
CAT# AFG-BRV-0944
Size: 1 Unit
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AffiAAV® rAAV-EF1a-EGFP-P2A-Cre-WPRE-hGH polyA
CAT# AFG-BRV-0942
Size: 1 Unit
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AffiAAV® rAAV-EF1a-H2B-Cre-WPRE-hGH polyA
CAT# AFG-BRV-0941
Size: 1 Unit
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AffiAAV® rAAV-EF1a-Cre-WPRE-hGH polyA
CAT# AFG-BRV-0939
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-XCaMP-B-WPRE-hGH polyA
CAT# AFG-BRV-0926
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-XCaMP-R-WPRE-hGH polyA
CAT# AFG-BRV-0925
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-XCaMP-Y-WPRE-hGH polyA
CAT# AFG-BRV-0924
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-GCaMP6m-Xc-WPRE-hGH polyA
CAT# AFG-BRV-0915
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-jRCaMP1a-WPRE-hGH polyA
CAT# AFG-BRV-0911
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-CaMPARI2-WPRE-pA
CAT# AFG-BRV-0882
Size: 1 Unit
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AffiAAV® rAAV-EF1a-DIO-jGCaMP7s-WPRE-hGH polyA
CAT# AFG-BRV-0859
Size: 1 Unit
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