rAAV Regulatory Elements

Recombinant adeno-associated virus (rAAV) vectors are widely used in gene therapy due to their safety, stable gene expression, and ability to transduce a variety of cell types. The efficiency and specificity of rAAV-based systems rely heavily on the regulatory elements embedded within the vector construct. These elements dictate transcription, translation, and gene expression levels, making them critical for therapeutic applications.

Key rAAV Regulatory Elements:
  • Inverted Terminal Repeats (ITRs):
    • Role: The ITRs, located at both ends of the rAAV genome, are crucial for replication, packaging, and integration of the vector DNA.
    • Application: They facilitate the formation of single-stranded DNA, the precursor for second-strand synthesis necessary for transgene expression.
  • Promoters:
    • Role: Promoters regulate the initiation of transcription. They can be:
      • Ubiquitous (e.g., CMV, EF1α): Drive expression in most cell types.
      • Tissue-specific (e.g., Syn1 for neurons): Enhance cell-type targeting and minimize off-target effects.
    • Application: Selection of promoters depends on the target tissue or disease model.
  • Polyadenylation (PolyA) Signals:
    • Role: Ensure stability of mRNA and efficient translation by signaling termination of transcription.
    • Application: Commonly used PolyA signals include SV40 or bGH PolyA for robust mRNA processing.
Applications of rAAV Regulatory Elements:
  • Gene Replacement Therapy:
    • Example: Delivering functional copies of genes in disorders like spinal muscular atrophy (SMA) or hemophilia.
  • Gene Silencing:
    • Usage of tissue-specific promoters for RNA interference or antisense oligonucleotides.
  • Oncolytic Therapies:
    • Leveraging tumor-specific promoters to selectively express cytotoxic genes in cancer cells.
  • CRISPR/Cas9 Delivery:
    • Utilizing rAAV vectors with precise regulatory elements to deliver genome-editing tools.
  • Neurological Diseases:
    • Targeting neural tissues using Syn1 or hGFAP promoters for disorders like Parkinson's disease or Huntington's disease.

rAAV regulatory elements are pivotal in designing safe, efficient, and targeted gene therapies. Their modular and tunable nature allows for tailored applications across various diseases, reinforcing their role in advancing modern medicine.

AffiAAV® rAAV-CMV-rox-stop-rox-mCherry-WPREs
CAT# AFG-BRV-0279
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE tight-fDIO-mCherry-WPREs
CAT# AFG-BRV-0277
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-fDIO-tdTomato-WPRE-hGH polyA
CAT# AFG-BRV-0276
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-fDIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0275
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-fDIO-TagBFP-WPRE-hGH polyA
CAT# AFG-BRV-0274
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® scAAV-hSyn-fDIO-EGFP-WPREs
CAT# AFG-BRV-0273
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-fDIO-EGFP-WPREs
CAT# AFG-BRV-0272
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-fDIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0271
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-fDIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0270
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-fDIO-BFP-WPRE-hGH polyA
CAT# AFG-BRV-0269
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-fDIO-tdTomato-WPRE-hGH polyA
CAT# AFG-BRV-0268
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-fDIO-axon-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0267
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-fDIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0266
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-fDIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0265
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-fDIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0264
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-fDIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0263
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-nEF1a-fDIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0262
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-nEF1a-fDIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0261
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-nEF1α-fDIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0260
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CHAT-DIO-EGFP-WPREs
CAT# AFG-BRV-0259
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-OT-DIO-mCherry-WPREs
CAT# AFG-BRV-0258
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-VGLUT2-DIO-EGFP-WPREs
CAT# AFG-BRV-0257
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-VGAT1-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0256
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-VGAT1-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0255
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-mDlx-DIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0254
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-mDLx-DIO-mCherry-WPREs
CAT# AFG-BRV-0253
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-mDlx-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0252
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-mDlx-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0251
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0250
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0249
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0248
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-3XFlag-WPREs
CAT# AFG-BRV-0246
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-Flag-WPREs
CAT# AFG-BRV-0245
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0244
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-tdTomato-WPRE-hGH polyA
CAT# AFG-BRV-0243
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-WPRE-hGH polyA
CAT# AFG-BRV-0242
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-NLS-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0241
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-FLEX-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0240
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-mcherry-mcherry-WPREs
CAT# AFG-BRV-0239
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0238
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-mCherry-WPREs
CAT# AFG-BRV-0237
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0236
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-EYFP-EYFP-WPREs
CAT# AFG-BRV-0235
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0234
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0233
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-H2B-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0232
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1α-DIO-H2B-mCherry-WPREs
CAT# AFG-BRV-0231
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-3XFlag-P2A-EGFP-WPREs
CAT# AFG-BRV-0230
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-ZsGreen1-WPREs
CAT# AFG-BRV-0229
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0228
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-H2B-Dsred-WPRE-hGH polyA
CAT# AFG-BRV-0227
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-3XFlag-WPREs
CAT# AFG-BRV-0226
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-3XMyc-WPRE-hGH polyA
CAT# AFG-BRV-0225
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-3XHA-WPRE-hGH polyA
CAT# AFG-BRV-0224
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-0223
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-Flag-WPREs
CAT# AFG-BRV-0218
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-Flag-WPREs
CAT# AFG-BRV-0217
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-H2B-EGFP-WPRE-pA
CAT# AFG-BRV-0216
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0215
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-EGFP-SV40 pA
CAT# AFG-BRV-0214
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1α-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0213
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1α-DIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0212
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0211
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-BFP-Flag-WPRE-bGH polyA
CAT# AFG-BRV-0210
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® scAAV-hSyn-DIO-EEGFP-WPREs
CAT# AFG-BRV-0209
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® scAAV-hSyn-DIO-mCherry-WPREs
CAT# AFG-BRV-0208
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-DIO-mScarlet-WPREs
CAT# AFG-BRV-0207
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-DIO-3XFlag-P2A-EGFP-WPREs
CAT# AFG-BRV-0206
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® scAAV-hSyn-DIO-EGFP-WPREs
CAT# AFG-BRV-0204
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-DIO-GFP-WPRE-hGH polyA
CAT# AFG-BRV-0203
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-DIO-mEGFP-WPRE-hGH polyA
CAT# AFG-BRV-0202
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0199
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-DIO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0198
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0197
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CaMKIIa-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0196
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CaMKIIa-DIO-EGFP-WPREs
CAT# AFG-BRV-0195
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CaMKIIa-DIO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0194
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-nEF1a-DIO-mCherry-WPREs
CAT# AFG-BRV-0193
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-nEF1a-DIO-3XFlag-WPREs
CAT# AFG-BRV-0192
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-nEF1a-DIO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0191
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-nEF1a-DIO-Flag-WPREs
CAT# AFG-BRV-0190
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-DO-EGFP-WPREs
CAT# AFG-BRV-0189
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-VGAT1-DO-tdTomato-DIO-EGFP-WPREs
CAT# AFG-BRV-0188
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CaMKIIa-DO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0187
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CaMKIIa-DO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0186
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DO-EGFP-WPREs
CAT# AFG-BRV-0185
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DO-tdTomato-WPRE-hGH polyA
CAT# AFG-BRV-0184
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DO-Dsred-WPRE-hGH polyA
CAT# AFG-BRV-0183
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DO-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-0182
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DO-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-0181
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DO-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-0180
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD