rAAV Regulatory Elements

Recombinant adeno-associated virus (rAAV) vectors are widely used in gene therapy due to their safety, stable gene expression, and ability to transduce a variety of cell types. The efficiency and specificity of rAAV-based systems rely heavily on the regulatory elements embedded within the vector construct. These elements dictate transcription, translation, and gene expression levels, making them critical for therapeutic applications.

Key rAAV Regulatory Elements:
  • Inverted Terminal Repeats (ITRs):
    • Role: The ITRs, located at both ends of the rAAV genome, are crucial for replication, packaging, and integration of the vector DNA.
    • Application: They facilitate the formation of single-stranded DNA, the precursor for second-strand synthesis necessary for transgene expression.
  • Promoters:
    • Role: Promoters regulate the initiation of transcription. They can be:
      • Ubiquitous (e.g., CMV, EF1α): Drive expression in most cell types.
      • Tissue-specific (e.g., Syn1 for neurons): Enhance cell-type targeting and minimize off-target effects.
    • Application: Selection of promoters depends on the target tissue or disease model.
  • Polyadenylation (PolyA) Signals:
    • Role: Ensure stability of mRNA and efficient translation by signaling termination of transcription.
    • Application: Commonly used PolyA signals include SV40 or bGH PolyA for robust mRNA processing.
Applications of rAAV Regulatory Elements:
  • Gene Replacement Therapy:
    • Example: Delivering functional copies of genes in disorders like spinal muscular atrophy (SMA) or hemophilia.
  • Gene Silencing:
    • Usage of tissue-specific promoters for RNA interference or antisense oligonucleotides.
  • Oncolytic Therapies:
    • Leveraging tumor-specific promoters to selectively express cytotoxic genes in cancer cells.
  • CRISPR/Cas9 Delivery:
    • Utilizing rAAV vectors with precise regulatory elements to deliver genome-editing tools.
  • Neurological Diseases:
    • Targeting neural tissues using Syn1 or hGFAP promoters for disorders like Parkinson's disease or Huntington's disease.

rAAV regulatory elements are pivotal in designing safe, efficient, and targeted gene therapies. Their modular and tunable nature allows for tailored applications across various diseases, reinforcing their role in advancing modern medicine.

AffiAAV® rAAV-VGLUT1-hChR2(H134R)-EYFP-WPREs
CAT# AFG-BRV-1136
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-OT-hChR2(H134R)-mCherry-WPREs
CAT# AFG-BRV-1135
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-GfaABC1D-hchR2(H134R)-EYFP-WPREs
CAT# AFG-BRV-1134
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-GFAP-hChR2(H134R)-EGFP-WPREs
CAT# AFG-BRV-1133
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-CaMKIIa-hChR2(H134R)-BFP-WPREs
CAT# AFG-BRV-1130
Size: 1 Unit
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AffiAAV® rAAV-CAG-hChR2(H134R)-EGFP-WPREs
CAT# AFG-BRV-1122
Size: 1 Unit
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AffiAAV® rAAV-EF1a-ChR2-EYFP-WPRE-hGH polyA
CAT# AFG-BRV-1118
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-CheRiff-eGFP-WPRE-bGH polyA
CAT# AFG-BRV-1116
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-HA-Dre-WPRE-hGH polyA
CAT# AFG-BRV-1115
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-mP2-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1113
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-OT-Flp-WPREs
CAT# AFG-BRV-1112
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D2-Flp-mCherry-WPRE-bGH polyA
CAT# AFG-BRV-1111
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D1-Flp-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1110
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TH-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1109
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-NPY-Flp-WPRE-bGH polyA
CAT# AFG-BRV-1104
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-NPY-Flp-WPREs
CAT# AFG-BRV-1103
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-VGAT2-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1102
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-VGAT1-Flp-WPREs
CAT# AFG-BRV-1101
Size: 1 Unit
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AffiAAV® rAAV-cFos-Flp-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1098
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-cFos-Flp-mCherry-WPRE-bGH polyA
CAT# AFG-BRV-1097
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE-Flp-BFP-WPRE-hGH polyA
CAT# AFG-BRV-1096
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRE tight-DIO-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1095
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CaMKIIa-Flp-WPRE-hGHpA
CAT# AFG-BRV-1094
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CaMKIIa-Flp-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1093
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® scAAV-hSyn-Flpo-WPREs
CAT# AFG-BRV-1092
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-hSyn-Flp-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1090
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hSyn-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1088
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-Flp-plus-WPRE-hGH polyA
CAT# AFG-BRV-1087
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-EF1α-DO-Flp-P2A-mCherry-WPREs
CAT# AFG-BRV-1085
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1a-DIO-Flp-WPREs
CAT# AFG-BRV-1082
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1α-DIO-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1081
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-EF1α-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1080
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAG-DIO-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1079
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® scAAV-CMV-DIO-Flp-WPREs
CAT# AFG-BRV-1078
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Flp-WPRE-hGH polyA
CAT# AFG-BRV-1077
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TRH-Cre-WPRE-hGH pA
CAT# AFG-BRV-1075
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® AAV-hALDH1L1-iCre-P2A-EGFP-WPRE-hGH Pa
CAT# AFG-BRV-1074
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-hALDH1L1-Cre-WPRE-PA
CAT# AFG-BRV-1073
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-SFFV-iCre-WPREs
CAT# AFG-BRV-1072
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-NEFH-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1071
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-5HT3R-Cre-WPREs
CAT# AFG-BRV-1070
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-SOX2-Cre-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1069
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-HypoCretin-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1068
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-GRP-Cre-P2A-EGFP-WPREs
CAT# AFG-BRV-1067
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-VIP-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1066
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-AVP-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1065
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-Penk-Cre-EGFP-WPREs
CAT# AFG-BRV-1064
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-Mecp2-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1063
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-KI67-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1062
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CS-CRM4-αMHC-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1061
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-cTNT-iCre-IRES2-tdTamato-polyA
CAT# AFG-BRV-1060
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-cTNT-EGFP-P2A-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1059
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TBG-EGFP-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1058
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TBG-Cre-EGFP-WPRE-pA
CAT# AFG-BRV-1057
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-TBG-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1056
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-Grm6-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1055
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CAR-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1054
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-RK-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1053
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-PTH-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1052
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CRH-Cre-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-1051
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CRH-Cre-P2A-EGFP-WPREs
CAT# AFG-BRV-1050
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CRH-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1049
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-OXTR-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1048
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® scAAV-mOXT-Cre-WPREs
CAT# AFG-BRV-1047
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-mOXT-Cre-P2A-mCherry-WPRE-hGH pA
CAT# AFG-BRV-1046
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-mOXT-Cre-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1045
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-mOXT-Cre-WPRE-hGH polyA
CAT# AFG-BRV-1044
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-OT-Cre-mCherry-WPREs
CAT# AFG-BRV-1043
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-Oxytocin-Cre-WPREs
CAT# AFG-BRV-1042
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D2-fDIO-Cre-mCherry-WPRE-hGH polyA
CAT# AFG-BRV-1041
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D2-Cre-mCherry-WPRE-bGH polyA
CAT# AFG-BRV-1040
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D2-Cre-EGFP-WPRE-bGH polyA
CAT# AFG-BRV-1039
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D2(ENK)-Cre-WPRE-bGH polyA
CAT# AFG-BRV-1038
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-DYN-Cre-WPREs
CAT# AFG-BRV-1037
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D1-fDIO-Cre-mCherry-WPRE-bGH polyA
CAT# AFG-BRV-1036
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D1-Cre-mCherry-WPRE-bGH polyA
CAT# AFG-BRV-1035
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-D1-Cre-EGFP-WPRE-hGH polyA
CAT# AFG-BRV-1034
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD