rAAV Regulatory Elements CBA

The CBA promoter (Cytomegalovirus enhancer fused to the chicken β-actin promoter) is a widely used hybrid promoter in recombinant adeno-associated virus (rAAV) vector systems. It combines elements of the CMV immediate-early enhancer and the chicken β-actin promoter, creating a robust and versatile regulatory element.

Structure and Composition:
  • CMV Enhancer:
    • A strong viral enhancer that drives high levels of transcription initiation.
  • Chicken β-actin Promoter:
    • A constitutive promoter derived from the chicken β-actin gene, ensuring broad and stable expression in various tissues.
  • Hybrid Architecture:
    • The combination of these two elements produces a promoter with both strength and flexibility, capable of supporting high transgene expression levels.
Features of the CBA Promoter:
  • Broad Expression Profile:
    • Active in a wide range of mammalian tissues and cell types, including neurons, hepatocytes, and muscle cells.
  • High Transcriptional Activity:
    • Delivers strong and sustained transgene expression, making it suitable for applications requiring robust protein production.
  • Minimal Silencing:
    • Unlike the standalone CMV promoter, the CBA promoter exhibits reduced susceptibility to epigenetic silencing in some cell types.
Applications in rAAV-Based Gene Therapy:
  • Neurological Disorders:
    • Drives transgene expression in neurons for conditions like Parkinson's disease and Huntington's disease.
  • Muscle Diseases:
    • Effective in skeletal and cardiac muscle cells for diseases such as Duchenne muscular dystrophy.
  • Liver-Targeted Therapies:
    • Suitable for hepatocyte transduction in diseases like hemophilia and metabolic disorders.

The CBA promoter is a cornerstone in rAAV vector design due to its strength, broad activity, and stability. Its inclusion in therapeutic constructs ensures reliable and efficient transgene expression, facilitating advancements in gene therapy.

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AffiAAV® rAAV-CBA-mCherry-P2A-hTau full-WPREs
CAT# AFG-BRV-0542
Size: 1 Unit
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