rAAV-Regulatory Elements FLAG

The FLAG tag is a short peptide sequence (DYKDDDDK) commonly used as an epitope tag in recombinant protein expression systems, including those involving rAAV vectors. It serves as a regulatory element for downstream protein detection and purification rather than influencing transcription or translation directly.

Function of the FLAG Tag in rAAV Systems:
  • Protein Detection:
    • The FLAG tag is recognized by highly specific antibodies, enabling easy detection of the expressed protein via techniques such as:
      • Western blotting
      • Immunofluorescence
      • Enzyme-linked immunosorbent assay (ELISA)
  • Protein Purification:
    • Facilitates affinity purification using anti-FLAG antibody-conjugated beads or columns.
  • Protein Tracking:
    • Acts as a molecular marker to study protein localization, trafficking, and interactions.
Design and Integration of FLAG Tags in rAAV Vectors:
  • N- or C-terminal Fusion:
    • The FLAG tag is fused to the N- or C-terminus of the therapeutic or experimental protein without significantly altering its structure or function.
  • Linker Usage:
    • Flexible linkers are often added between the tag and the protein to avoid steric hindrance and maintain protein activity.
Applications of FLAG Tag in rAAV-Based Gene Therapy and Research:
  • Therapeutic Protein Validation:
    • Ensures proper expression and structural integrity of the therapeutic protein.
  • Biological Research:
    • Tracks protein expression and localization in tissues or cell cultures.
  • Protein Interaction Studies:
    • Identifies interacting partners through co-immunoprecipitation (Co-IP) or pull-down assays.
  • Quality Control in Production:
    • Facilitates monitoring during the production of rAAV-delivered proteins for clinical applications.

The FLAG tag is a simple yet powerful tool in rAAV systems for monitoring and purifying expressed proteins. Its versatility makes it an invaluable element in both therapeutic and research contexts.

AffiAAV® rAAV-CMV-DIO-3XFlag-WPREs
CAT# AFG-BRV-0246
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-DIO-Flag-WPREs
CAT# AFG-BRV-0245
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-GFAP-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-0092
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-3×Flag-WPRE-hGH polyA
CAT# AFG-BRV-0072
Size: 1 Unit
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AffiAAV® rAAV-CMV-Flag-Flag-WPREs
CAT# AFG-BRV-0063
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Flag-WPREs
CAT# AFG-BRV-0061
Size: 1 Unit
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AffiAAV® rAAV-CMV-mCherry-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-0059
Size: 1 Unit
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AffiAAV® rAAV-CMV-EGFP-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-0057
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-CAG-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-0044
Size: 1 Unit
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AffiAAV® rAAV-GFAP-Cre-3XFlag-WPRE-hGH polyA
CAT# AFG-BRV-1012
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiAAV® rAAV-CMV-Cre-Flag-WPREs
CAT# AFG-BRV-0931
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
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AffiAAV® rAAV-EF1α-CreERT-3XFlag-WPREs
CAT# AFG-BRV-0369
Size: 1 Unit
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AffiAAV® rAAV-GFAP-rTTA-3XFlag-WPREs
CAT# AFG-BRV-0364
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD