rAAV-Regulatory Elements mCherry
mCherry is a red fluorescent protein (RFP) derived from the Discosoma sp. fluorescent proteins. It is widely used in recombinant adeno-associated virus (rAAV) systems as a reporter gene for tracking, visualization, and monitoring gene expression and cellular processes.
Features of mCherry:
- Bright Fluorescence:
- Excitation/Emission Peaks: 587 nm/610 nm, producing bright red fluorescence.
- Monomeric Nature:
- Unlike some fluorescent proteins, mCherry is monomeric, making it suitable for fusion with other proteins without interfering with their function.
- Stability:
- Highly stable in a wide range of pH conditions and resistant to photobleaching.
- Fast Maturation:
- Quickly matures into a fluorescent state, enabling real-time visualization shortly after expression.
Applications of mCherry in rAAV Systems:
- Reporter Gene:
- Acts as a visual marker for transgene expression, allowing researchers to confirm the efficiency of rAAV delivery and expression in vivo or in vitro.
- Cell Tracking:
- Used to monitor cell fate, migration, and lineage in live imaging studies.
- Fusion Tag:
- Fused to other proteins to study protein localization, trafficking, and interaction.
- Tissue-Specific Expression Studies:
- Incorporated into rAAV constructs with tissue-specific promoters to observe gene expression in targeted organs (e.g., brain, liver, retina).
Advantages of mCherry in rAAV-Based Research:
- Low Toxicity:
- Minimal cytotoxic effects, even during prolonged expression.
- Non-Interfering:
- Monomeric structure ensures compatibility as a fusion tag without disrupting host protein function.
- Compatibility:
- Effective in both live-cell imaging and fixed-tissue studies across various model systems.
mCherry is an indispensable tool in rAAV systems, providing a versatile and reliable means to visualize and quantify gene expression. Its bright fluorescence, stability, and adaptability have made it a cornerstone in gene therapy research and advanced biological studies.
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