rAAV-Regulatory Elements T24

The T2A peptide is a self-cleaving peptide derived from the Thosea asigna virus. It is widely used in recombinant adeno-associated virus (rAAV) systems to enable co-expression of multiple genes from a single promoter, ensuring efficient and balanced production of therapeutic or experimental proteins.

Key Features of T2A:

  • Self-Cleaving Property:
    • T2A induces ribosomal skipping during translation, creating separate proteins from a single mRNA transcript.
    • The resulting proteins are expressed as individual entities, with only a few residual amino acids attached to the C-terminus of the upstream protein.
  • Short Sequence:
    • The T2A peptide is only 18–20 amino acids long, making it compact and easy to incorporate into rAAV vectors:
      • Sequence: GSGEGRGSLLTCGDVEENPGP

Applications in rAAV Systems:

  • Multigene Expression:
    • Enables the expression of multiple therapeutic genes or protein subunits from a single promoter.
    • Example: Co-expression of a reporter (e.g., GFP) and a therapeutic protein.
  • CRISPR/Cas9 Delivery:
    • Facilitates the expression of Cas9 nuclease and guide RNA processing enzymes from a single vector.
  • Gene Therapy:
    • Allows the co-delivery of two or more therapeutic proteins, such as enzymes or growth factors, in diseases requiring multi-protein approaches.
  • Bispecific Antibodies:
    • Used in the production of bispecific or multispecific antibodies by expressing separate antibody chains from a single construct.

Advantages of T2A in rAAV Systems:

  • Compact Design:
    • Small size conserves the limited packaging capacity of rAAV vectors (~4.7 kb).
  • Balanced Expression:
    • Ensures proportional expression of linked proteins, reducing variability.
  • Single Promoter:
    • Simplifies vector design and avoids the need for multiple promoters, which can interfere with each other.

The T2A peptide is a powerful and efficient tool in rAAV vector design, enabling flexible and balanced expression of multiple genes from a single promoter. Its compact size and high cleavage efficiency make it indispensable for gene therapy, genome editing, and advanced biological research.

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AffiAAV® rAAV-GFAP-EGFP-T2A-TVA-WPRE-hGH polyA
CAT# AFG-BRV-0512
Size: 1 Unit
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