rAAV-Regulatory Elements T24
The T2A peptide is a self-cleaving peptide derived from the Thosea asigna virus. It is widely used in recombinant adeno-associated virus (rAAV) systems to enable co-expression of multiple genes from a single promoter, ensuring efficient and balanced production of therapeutic or experimental proteins.
Key Features of T2A:
- Self-Cleaving Property:
- T2A induces ribosomal skipping during translation, creating separate proteins from a single mRNA transcript.
- The resulting proteins are expressed as individual entities, with only a few residual amino acids attached to the C-terminus of the upstream protein.
- Short Sequence:
- The T2A peptide is only 18–20 amino acids long, making it compact and easy to incorporate into rAAV vectors:
- Sequence: GSGEGRGSLLTCGDVEENPGP
- The T2A peptide is only 18–20 amino acids long, making it compact and easy to incorporate into rAAV vectors:
Applications in rAAV Systems:
- Multigene Expression:
- Enables the expression of multiple therapeutic genes or protein subunits from a single promoter.
- Example: Co-expression of a reporter (e.g., GFP) and a therapeutic protein.
- CRISPR/Cas9 Delivery:
- Facilitates the expression of Cas9 nuclease and guide RNA processing enzymes from a single vector.
- Gene Therapy:
- Allows the co-delivery of two or more therapeutic proteins, such as enzymes or growth factors, in diseases requiring multi-protein approaches.
- Bispecific Antibodies:
- Used in the production of bispecific or multispecific antibodies by expressing separate antibody chains from a single construct.
Advantages of T2A in rAAV Systems:
- Compact Design:
- Small size conserves the limited packaging capacity of rAAV vectors (~4.7 kb).
- Balanced Expression:
- Ensures proportional expression of linked proteins, reducing variability.
- Single Promoter:
- Simplifies vector design and avoids the need for multiple promoters, which can interfere with each other.
The T2A peptide is a powerful and efficient tool in rAAV vector design, enabling flexible and balanced expression of multiple genes from a single promoter. Its compact size and high cleavage efficiency make it indispensable for gene therapy, genome editing, and advanced biological research.
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